Professor Utonium planned to start a family of his own by creating three daughters to raise, with the basic ingredients of sugar, spice, and everything nice. Unfortunately, the professor's clumsiness caused him to accidentally add Chemical X to the ingredients, which resulted in the creation of Blossom, Bubbles, and Buttercup. Known as The Powerpuff Girls, the three girls quickly become well-known as the heroes of the City of Townsville.
- The Powerpuff Girls is one of the two Cartoon Network owned franchises of the game with the other being Adventure Time. Teen Titans Go! also airs on Cartoon Network, but is not owned by the channel.
- The Powerpuff Girls appeared in the Teen Titans Go! crossover episode "TTG v PPG".